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Spectrometer SM 9000

The new Spectrometer SM 9000 was developed for high-resolution, fiber optic spectroscopy applications in the UV/VIS to NIR range. It can be used as a stand-alone instrument or as a substantial enhancement of other PSI instruments to analyze spectral composition of irradiance. Ultra-high sensitivity of the instrument allows measuring fluorescence emission spectra of individual cells when combined with µ-FluorCam. The spectra can be measured in the range from UV (200 nm) to NIR (980 nm) with FWHM bandwidth of 3.5 nm. Fast recording can capture as many as 100 spectra per second with data resolution of 16 bits.
  • Ultra-high sensitivity.
  • Suitable for microspectrophotometry.
  • FWHM bandwidth of 3.5 nm.
  • Small-footprint, modular, robust, and thermally stable.
  • Very low heat generation.
  • 10 µs - 10 ms duration of flashes.
  • Variable exposure time, from 1 millisecond to minutes.
  • Kinetic spectra (max. 100 per second).
  • Optical Entrance
    diameter 0.5 NA = 0.22 mounted in SMA-coupling, dismountable
  • Entrance slit
    70 µm x 1400 µm (optical entrance)
  • Grating
    Flat-field correction
  • Spectral range
    200 nm - 980 nm
  • Wavelength accuracy absolute
    < 0.5 nm
  • Reproducibility
    < 0.1 nm
  • Temperature - induced drift
    <0.01 nm/K
  • Spectral distance of pixel
    Δλ pixel ~ 0.8 nm
  • (FWHM: Full width half maximum)
    Δλ < 3 ...4 nm (UV-NIR Version)
  • Straylight
    0.1 % measured at 340 nm with deuterium lamp (transmission of NaNO2 solution, 50 g/l, 1 cm)
  • CCD Array
    Thermoelectrically cooled Hamamatsu S 7031
  • Number of pixels
    1044 x 64
  • Dimensions of pixels
    24 x 24 mm2
  • System data
    16 Bit AD conversion
  • Noise
    2...4 count standard deviation
  • CHEREGI O., KOTABOVÁ E., PRÁŠIL O., ET AL. (2015): Presence of state transitions in the cryptophyte alga Guillardia theta . Journal of Experimental Botany. Volume 66. Pages 6461–6470.DOI: 10.1093/jxb/erv362
  • LI, G., BROWN, C. M., JEANS, J. A., ET AL. (2015): The nitrogen costs of photosynthesis in a diatom under current and future pCO2. New Phytologist. Volume 205. Pages 533–543. DOI:10.1111/nph.13037
  • KOTABOVÁ, E., JAREŠOVÁ, J., KAŇA, R., ET AL. (2014): Novel type of red-shifted chlorophyll a antenna complex from Chromera velia. I. Physiological relevance and functional connection to photosystems. Biochimica et Biophysica Acta – Bioenergetics. Volume 1837. Pages 734-743. DOI: 10.1016/j.bbabio.2014.01.012
  • ŠEBELA D., OLEJNÍČKOVÁ J., SOTOLÁŘ R. ET AL. (2014): The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition. BBA 1817. Pages 1237-1247. DOI: 10.1016/j.bbabio.2012.02.024
  • KÁŇA R., KOTABOVÁ E., SOBOTKA R., ET AL. (2012): Non-Photochemical quenching in Cryptophyte Alga Rhodomonas salina is located in chlorophyll a/c antennae. PLOS ONE . Volume 7. DOI: 10.1371/journal.pone.0029700
  • KÁŇA R., KOTABOVÁ E., KOMÁREK O., ET AL. (2012): The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition. BBA 1817. Pages 1237-1247. DOI: 10.1016/j.bbabio.2012.02.024
  • ŠEBELA D., OLEJNÍČKOVÁ J., SOTOLÁŘ R. ET AL. (2014): The slow S to M fluorescence rise in cyanobacteria is due to a state 2 to state 1 transition. BBA 1817. Pages 1237-1247. DOI: 10.1016/j.bbabio.2012.02.024
  • QUIGG A., KOTABOVÁ E., JARESOVÁ J., ET AL. (2012): Photosynthesis in Chromera velia represents a simple system with high efficiency. PLOS ONE. Volume 7. DOI:10.1371/journal.pone.0047036
  • ŠEBELA D., OLEJNÍČKOVÁ J., ŽUPČANOVÁ A. ET AL. (2012): Response of grapevine leaves to Plasmopara viticola infection by means of measurement of reflectance and fluorescence sinals. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis. Volume 60. Pages 229-238. DOI: 10.11118/actaun201260080229
  • KÁŇA R., PRÁŠIL O., KOMÁREK O., ET AL. (2009): Spectral characteristic of fluorescence induction in a model cyanobacterium Synechococcus sp. (PCC 7942). Biochimica et Biophysica Acta. Volume 1787. Pages 1170-1178. DOI: 10.1016/j.bbabio.2009.04.013
  • SUGGETT D. J., STAMBLER N., PRÁŠIL O. ET AL.((2009): Nitrogen and phosphorus limitation of oceanic microbial growth during spring in the Gulf of Aqaba. Aquat Microb Ecology. Volume 56. Pages 227-239. DOI:10.3354/ame01357

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