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Handy GFPCam FluorCam

Handy GFPCam FC 1000-H/GFP is a modified version of the popular Handy FluorCam. Its unique construction allows both time resolved chlorophyll fluorescence imaging and GFP imaging. Handy GFPCam is a lightweight, portable system designed for imaging of leaves, small plants, leaf segments, mosses, lichens, seeds, roots, tissues on plates or algal colonies both in a field and laboratory. The typical size of an investigated object is up to 3.5 × 4.6 cm. The Handy GFPCam is an easily portable system, it can be powered from the power supply or it can run on batteries provided in a convenient bag carried on the shoulder.

Handy GFPCam FC 1000-H/GFP is typically produced with red-orange flashing LED panels (620 nm) and blue actinic/saturating pulse LED panels (455 nm). Optionally, the system can be equipped with four additional far-red LEDs. Handling of larger leaves (e.g., tobacco) is simplified if an additional leaf clip with gentle lock/release mechanism is mounted.

Handy GFPCam generates images of fluorescence signal at any moment of the experiment and presents them using a false color scale. Full kinetic analysis is available. In all applications, the camera allows imaging of fluorescence transients that are induced by actinic light or by saturating flashes. The timing and amplitude of actinic irradiance are determined by user-defined protocols. The Handy GFPCam is delivered with a laptop computer preinstalled with a comprehensive software package comprising full system control, Wizard with the most frequently used experimental protocols, data acquisition and image processing. For an experienced professional, the software offers a sophisticated programming language that can be used for designing novel timing and measuring sequences.

  • Combined imaging of GFP and chlorophyll fluorescence
  • Both lab and field applications
  • Portable and compact (21.5 cm x 13.5 cm x 13.5 cm)
  • AC & battery powered
  • Coming with a high-end computer and user-friendly software
  • High-resolution CCD camera (included)
  • Imaged area: 35 x 46 mm
  • GFP-Cam Image
  • Steady-state GFP fluorescence
  • FV/FM
  • Kautsky induction
  • Quenching analysis
  • Light curve
  • Handy FluorCam
  • Measured parameters: FO, FM, FV, FO', FM', FV', FT
  • More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, ETR (via Light Curve protocol), PAR-absorptivity coefficient, and many others
  • 52 Images in One Measurement
    Images of More Than 50 Calculated Parameters in One Measurement
  • Screening for GFP-containing GMOs
  • Transgene expression & localization
  • Plant-microbe interactions
  • Bacterial-protozoan interactions
  • Screening for photosynthetic performance
  • Stress resistance studies
  • Arabidopsis Image Captured by the GFPCam
    Arabidopsis Image Captured by the GFPCam:
    - GFP mutant expresses in colors
    - wildtype expresses in gray (bottom)
  • Leaves, small plants, stems, seeds, roots
  • Calluses, seedlings on Petri plates
  • Bacteria, yeasts, cyanobacteria, green algae
  • Small animals
  • Sample size up to 35 x 46 mm
  • Corn Kernel Image
    Corn Kernel Image
  • Imaged area: 35 x 46 mm
  • Excitation light colors:
    - measuring pulses: orange (620 nm), blue (455 nm)
    - actinic / saturating light: white – blue (455 nm)
    - far-red 735 nm
  • Saturating pulse intensity:
    - blue: up to 4,900 µmol(photons)/m².s
  • Actinic light intensity:
    - blue: approx. 1,400 µmol(photons)/m².s
  • Light regime:
    - Static or sinus form (hub connected)
  • Intended for combined measurement of ChlF and detection of weak steady-state fluorescence signals (such as GFP) with long integration times or applications where the higher spatial resolution is of highest importance (microscopy)
  • Progressive scan CCD, 1.4 M pixel sensor
  • A/D: 16 bit (65,536 grey levels)
  • 6.45 µm x 6.45 µm pixel size
  • Two operating modes:
    - video (ChlF kinetics measurement),
    - snapshot (long integration time for FPs detection)
  • Resolution:
    1360 x 1024 pixels; 20 images per second
  • Binning option: 2x2 - 680 x 512 pixels, 20 images per second
  • Interface connector: Gigabit ethernet
  • Fully automated control of the whole FluorCam system
  • Image acquisition via automated experimental protocols:
    - numerous predefined protocols
    - possibility to create user defined protocols via a program Wizard
    - multiple (automatically repeated) experiments
    - Barcode reader support
  • Image processing tools:
    - automatic or manual image segmentation – e.g., labeling of individual plants
    - analysis of kinetic data from all samples within the field of view
    - numerous image manipulation tools
    - export to text file, avi, bmp or raw data formats
  • Windows 2000, XP, Vista, W7 compatible*
  • Corn Kernels Captured by the GFPCam
    Corn Kernels Captured by the GFPCam:
    - GFP variant in the upper lines
    - control kernels in the bottom lines
* Windows 2000, Windows XP, Vista, and W7 are trademarks of Microsoft Corporation.
  • Fluorescence Parameters:
    - Measured parameters: FO, FM, FV, FO', FM', FV', FT
    - More than 50 calculated parameters: FV/FM, FV'/FM', PhiPSII , NPQ, qN, qP, Rfd, PAR-absorptivity coefficient, and many others
  • Excitation Light Sources:
    - Measuring pulses: orange (620 nm), blue (455 nm)
    - Actinic / saturating light: white - blue (455 nm)
  • Saturating Pulse Intensity:
    Blue: up to 4,900 µmol(photons)/m².s
  • Actinic Light Intensity:
    Blue: approx. 1,400 µmol(photons)/m².s
  • Light Regime:
    Static or sinus form (hub connected)
  • Custom-Defined Protocols:
    Variable timing, special language and scripts
  • CCD Detector Wavelength Range:
    400 – 1000 nm
  • CCD Format:
    1360 x 1024 pixels (TOMI-2 camera)
  • Pixel Size:
    6.45 µm x 6.45 µm (TOMI-2)
  • A/D Bit Resolution:
    16 bit (65,536 grey levels)
  • Spectral Response:
    QE max at 540 nm (~70 %), 50 % roll-off at 400 nm and 650 nm
  • Read-out Noise:
    Less than 12 electrons RMS - typically only 10 electrons
  • Full-Well Capacity:
    Greater than 70,000 electrons (unbinned)
  • Imaging Frequency:
    Maximum 50 frames per second
  • Bios:
    Upgradeable firmware
  • Interface Connector:
    Gigabit Ethernet
  • GFPCam Weight:
    1.8 kg
  • Leaf-Clip Weight:
    0.2 kg
  • Power Supply Weight:
    2.5 kg
  • GFPCam Stand Weight:
    1.5 kg
  • Notebook Weight (incl. all accessories):
    3.5 kg
  • Power Input:
    Max. 200 W
  • Electrical:
    90 – 260 V
  • Leal M.C. et al (2014): Marine Ecology (2014): 1-13, doi: 10.1111/maec.12164

Pricing

  • Orders and Payments
  • Handy GFPCam FC 1000-H/GFP
    16.990,- €
  • Leaf Clip
    390,- €
  • Ultra-Light Tripod *
    490,- €
  • Battery Pack *
    490,- €
  • Advanced Multiple Function for FluorCam Software
    400,- €
  • Advanced Module for Automatic and Programmable Image Acquisition and Data Management
    5.990,- €
  • Additional Protective Glasses *
    19,- €

Optional Features and Accessories

Ultra-Light Tripod
The tripod stand is a very useful accessory for field experiments. Its robust and flexible construction offers needed stability during imaging operations. The stand is water resistant and its variable height allows imaging of samples at different levels within the vegetation. Height up to 1.5 m; weight 3.3 kg; includes a bag.


Leaf Clip
The leaf clip simplifies the process of collecting fluorescence images on vegetation. The unique design includes a gentle lock/release system for holding the target leaf in place without detachment or excessive disturbance. It also allows for dark adaptation.


Battery Pack
A useful supplement for comfortable field measurements. Weight: 3.6 kg. Size: 22 x 20 x 13 cm.


Additional Protective Glasses
Radiation safety glasses protecting against excessive LED radiation – equipped with side cover and protective filters. One pair of glasses is provided with the device free of charge.


Advanced Multiple Function for FluorCam Software
Software upgrade enabling multiple scripting functions for automatic initiation and measurement of scheduled FluorCam protocols and for automatic data storage. Advanced multiple function feature allows to define time of protocol initiation with user-defined protocol, apply user defined mask for feature segmentation, automatically subtract the background based on the mask definition and analyze the measured data. The are stored as TAR files with time and date description. Useful for circadian cycle study.


Advanced Module for Automatic and Programmable Image Acquisition and Data Management
Comprehensive SW package for system control, data acquisition, image analysis and data base configuration. User friendly graphical interface PlantScreenTM Scheduler is designed to control hardware system components actions and to design experiments with an extremely high level of flexibility. Scheduling assistant implements calendar function and is designed for visualization, planning and management of the individual experiments. Complex protocols can be easily designed including dark/light adaptation schemes and executed based on user defined actions. All acquired imaging data are stored in an SQL database, processed and available for inspection and further analysis in range of seconds after recording via user-friendly graphical interface. It is an excellent tools for rapid and easy data browsing, grouping, analysis, user-defined data reprocessing and export. Key features are: straightforward access to analyzed parameters and acquired images over the time series of measurements, graphical display of trends for individual or multiple parameters, basic statistical analysis, visualization of time series image data for selected parameters and easy export function for numeric and image-based data for user defined sets of experiments. Multiple clients can be connected to the database, with different privileges assigned based on a built-in authentication mechanism.

Download

Software
FluorCam 6
OS: Win2000/XP
Language: English
Size: 6.3 MB


FluorCam 7
OS: Win XP/Vista/Seven (32bit)
Language: English
Size: 6.4 MB


USB driver for FluorCam
OS: Win2000/XP
Language: English
Size: 10 KB


USB driver for FluorCam
OS: Win Vista/Seven
Language: English
Size: 10 KB


Documents
FluorCam Manual
Type: PDF
Language: English
Size: 10.6 MB


FluorCam Quick Start Guide
Type: PDF
Language: English
Size: 428 KB


NDVI Operation Manual
Type: PDF
Language: English
Size: 1.4 MB


Multicolor Micro-FluorCam Manual
Type: PDF
Language: English
Size: 7.7 MB


Installation Manual for FluorCam FC-800-D-3535
Type: PDF
Language: English
Size: 1.2 MB


LED Spectra
Type: PDF
Language: English
Size: 121 KB


FluorCam Handy Brochure
Type: PDF
Language: English
Size: 215 KB


FluorCam Leaf-Clip Brochure
Type: PDF
Language: English
Size: 169 KB


Micro-FluorCam Brochure
Type: PDF
Language: English
Size: 212 KB


Handy GFPCam Brochure
Type: PDF
Language: English
Size: 173 KB


FluorCam Device Line - List of References
Type: PDF
Language: English
Size: 769 KB


Driver Installation
Type: PDF
Language: English
Size: 334 KB


Other FluorCams

Open FC 800-O/1010

Open FluorCam FC 800-O is a highly modular instrument with flexible geometry. The LED panels and the light source generating saturating flashes can be arranged at various angles and distances from the sample. Also, the position of the camera relative to the sample is adjustable.


Closed FC 800-C

Closed FluorCam FC 800-C represents a highly innovative, world-wide used system of multispectral kinetic fluorescence imaging. It consists of a CCD camera and four fixed LED panels. The LED panels provide uniform irradiance over samples up to 13 x 13 cm – suitable for small plants, detached leaves, algal dilutions, etc. The system allows dark adaptation.


Closed GFPCam FC 800-C/1010GFP

Closed GFPCam FC 800-C/1010GFP serves for various applications using green fluorescent proteins in molecular and cellular biology. The device is equipped with a motorized, software-controlled filter wheel, which provides quick and simple transition between the GFP and chlorophyl fluorescence detection and imaging.


Handy FC 1000-H

Handy FluorCam FC 1000-H is a portable system designed for kinetically resolved fluorescence imaging of leaves and small plants both in a field and laboratory. The whole instrument can be carried in a convenient bag over the shoulder and can run on batteries.


Handy GFPCam FC 1000-H/GFP

Handy GFPCam FC 1000-H/GFP is a modified version of the popular Handy FluorCam. Its unique construction allows both GFP imaging and chlorophyll fluorescence imaging. It can be favorably used for lab and field experiments.


Handy - Leaf Chamber FC 1000-LC

Leaf Chamber Fluorcam FC 1000-LC is a unique system that allows you to conduct chlorophyll fluorescence imaging on a leaf maintained in a leaf chamber, while measuring leaf gas exchange at the same time. The Fluorcam unit can be attached to leaf chambers provided by most manufacturers of leaf gas exchange equipment.


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